各位老师和同学：

大家好，2024年10月24日（星期四），化学生物学与生物技术学院邀请了威斯康星大学麦迪逊分校药学院和化学系李灵军教授到访我院并进行通过发展基于质谱的新方法推动生物医学研究进展的讲座，欢迎大家参加！

嘉    宾：李灵军 教授

嘉宾单位：威斯康星大学麦迪逊分校药学院和化学系

主    题：通过发展基于质谱的新方法推动生物医学研究进展

时    间：2024年10月24日（星期四）15：30-17：00

地    点：C105

邀请人：高晋君

讲座摘要：

Comprehensive characterization of all signaling molecules in a biological system with chemical, spatial and temporal information is often critical to deciphering their functional roles, yet it poses a daunting challenge. In this presentation, I will present our recent progress on the development of a multi-faceted mass spectrometry (MS)-based analytical platform to probe neuronal signaling with enhanced sensitivity and selectivity.  By combining chemical labeling, micro-scale separation, and tandem MS sequencing techniques, we discovered more than 300 novel neuropeptides in several model organisms.  Moreover, both mass spectrometric imaging (MSI) technology and in vivo microdialysis sampling tools have been developed and implemented to follow neuropeptide distribution and secretion with unprecedented details.  Additionally, several in situ chemical derivatization strategies have been developed to enable spatial mapping of various biomolecules including lipids and glycans in complex biological samples, such as human cell lines and cancer tissue samples.  Recent progress towards single-cell lipidomics enabled by dual-polarity ionization and ion mobility MS imaging will be highlighted as well.

Furthermore, we are developing multiplexed isobaric and isotopic tagging strategies to discover, identify and evaluate candidate biomarkers of Alzheimer’s disease (AD) in cerebrospinal fluids (CSFs) obtained from asymptomatic cognitively-healthy middle-aged adults, older cognitively-normal adults, and patients with mild cognitive impairment (MCI) and AD.  A large-scale comparative glycoproteomic analysis via the 12-plex DiLeu (N,N-dimethyl leucine) tagging strategy revealed distinct glycosylation patterns and dynamic changes of certain glycoforms in CSF samples collected from the control, MCI, and AD patients. Additionally, we report on a multiplexed quantitation method for simultaneous proteomics and amine metabolomics analyses via nanoflow reversed phase LC-MS/MS, exploiting mass defect-based DiLeu (mdDiLeu) labeling.  Several on-going efforts and future perspectives provided by these enabling technologies will be highlighted and discussed.

嘉宾简介：

李灵军教授是威斯康星大学麦迪逊分校药学院和化学系Charles Melbourne Johnson杰出讲座教授，并获该校Vilas杰出成就教授称号。李灵军教授是享誉世界的分析化学家和质谱学家,为相关领域技术的发展做出了卓越的贡献，先后被授予分析化学个人成就奖（匹兹堡会议）、美国质谱学会研究奖、2014年美国质谱学会Biemann Medal奖等荣誉，入选2016年分析科学家Power List-全球50位最有影响力的女分析化学家, 及多次入选Power List-全球最有影响力的分析化学家，并获得中国教育部“长江学者奖励计划”讲座教授称号。李灵军教授现任美国质谱学会杂志（Journal of The American Society for Mass Spectrometry）副主编和质谱综述（Mass Spectrometry Reviews）分析与生物分析化学(Analytical and Bioanalytical Chemistry)国际编委会成员，以及北美华人质谱学会董事会主席。

请注意： 本次讲座需要生物和化学方向学生签到！